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range protein marker rpn756  (GE Healthcare)


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    GE Healthcare range protein marker rpn756
    Range Protein Marker Rpn756, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/range protein marker rpn756/product/GE Healthcare
    Average 91 stars, based on 38 article reviews
    range protein marker rpn756 - by Bioz Stars, 2026-03
    91/100 stars

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    Immunoblotting with antiribosomal P proteins. (A) Identification of the cellular ribosomal P proteins (proteins of the 60S subunit of the ribosome) by Western blot using human antiribosomal P protein antiserum. P0 is 38 kDa, P1 is 19 kDa, and P2 is 17 kDa. Ribosomes purified from 293T cells were used as positive control (lanes 1 to 3, i.e., 250, 100, and 50 ng of proteins, respectively). An equal amount of virions is loaded on each lane, as determined by MuLV Gag or CA quantification by immunoblot using a p30CA antiserum (example in panel B). Lane 4, PR− MuLV; lane 5, C17−; and lane 6, C37−. Lanes 8 and 11, MuLV wild type; lane 9, NC(Δ16-23); and lane 12, C39S. The mock preparations correspond to the same volume of culture media as for C37− (lane 7) or NC(Δ16-23) virions (lane 10). wt, wild type. (B) Western blot of several samples used in panel A, probed with a p30CA antiserum. Lane M, protein molecular weight marker <t>RPN756</t> (Amersham Pharmacia) (in kilodaltons). Lane 1, wild-type MuLV; lanes 2, 3, and 4 are PR−, C17−, and C37− mutants, respectively.
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    Immunoblotting with antiribosomal P proteins. (A) Identification of the cellular ribosomal P proteins (proteins of the 60S subunit of the ribosome) by Western blot using human antiribosomal P protein antiserum. P0 is 38 kDa, P1 is 19 kDa, and P2 is 17 kDa. Ribosomes purified from 293T cells were used as positive control (lanes 1 to 3, i.e., 250, 100, and 50 ng of proteins, respectively). An equal amount of virions is loaded on each lane, as determined by MuLV Gag or CA quantification by immunoblot using a p30CA antiserum (example in panel B). Lane 4, PR− MuLV; lane 5, C17−; and lane 6, C37−. Lanes 8 and 11, MuLV wild type; lane 9, NC(Δ16-23); and lane 12, C39S. The mock preparations correspond to the same volume of culture media as for C37− (lane 7) or NC(Δ16-23) virions (lane 10). wt, wild type. (B) Western blot of several samples used in panel A, probed with a p30CA antiserum. Lane M, protein molecular weight marker RPN756 (Amersham Pharmacia) (in kilodaltons). Lane 1, wild-type MuLV; lanes 2, 3, and 4 are PR−, C17−, and C37− mutants, respectively.

    Journal:

    Article Title: Murine Leukemia Virus Nucleocapsid Mutant Particles Lacking Viral RNA Encapsidate Ribosomes

    doi: 10.1128/JVI.76.22.11405-11413.2002

    Figure Lengend Snippet: Immunoblotting with antiribosomal P proteins. (A) Identification of the cellular ribosomal P proteins (proteins of the 60S subunit of the ribosome) by Western blot using human antiribosomal P protein antiserum. P0 is 38 kDa, P1 is 19 kDa, and P2 is 17 kDa. Ribosomes purified from 293T cells were used as positive control (lanes 1 to 3, i.e., 250, 100, and 50 ng of proteins, respectively). An equal amount of virions is loaded on each lane, as determined by MuLV Gag or CA quantification by immunoblot using a p30CA antiserum (example in panel B). Lane 4, PR− MuLV; lane 5, C17−; and lane 6, C37−. Lanes 8 and 11, MuLV wild type; lane 9, NC(Δ16-23); and lane 12, C39S. The mock preparations correspond to the same volume of culture media as for C37− (lane 7) or NC(Δ16-23) virions (lane 10). wt, wild type. (B) Western blot of several samples used in panel A, probed with a p30CA antiserum. Lane M, protein molecular weight marker RPN756 (Amersham Pharmacia) (in kilodaltons). Lane 1, wild-type MuLV; lanes 2, 3, and 4 are PR−, C17−, and C37− mutants, respectively.

    Article Snippet: Lane M, protein molecular weight marker RPN756 (Amersham Pharmacia) (in kilodaltons).

    Techniques: Western Blot, Purification, Positive Control, Molecular Weight, Marker